Although the seed region of siRNA is implicated in the miRNAlike offtarget effect, only some mRNAs, with this type of sequence complementarity, are silenced by siRNA By analogy, miRNAs regulate the expression of multiple mRNAs through binding of the seed region to the target 3′UTR, but not all mRNAs with the same degree of sequence complementarity are the targets of a givenThe nonseed region of siRNA was found to be subdivided into four domains, in which two nucleotide pairs (positions 13 and 14) were replaceable with cognate deoxyribonucleotides without reducing RNAi activity However, RNA sequences at positions 912 and 1518 were essential for effective gene silencing,Signature transcript 39 UTRs contain sequence complementarity to siRNA seed regions The indicated siRNAs were transfected into HeLa cells Gene expression signatures (pvalue #
Passenger Strand Cleavage Facilitates Assembly Of Sirna Into Ago2 Containing Rnai Enzyme Complexes Cell
